Plant genetic resources (PGR) play a crucial role in supporting the livelihoods of humanity. Our research focuses on the sustainable utilization and conservation of these resources, with a primary emphasis on various crops such as fruits (e.g. pineapple, bananas, plantain, sisrè berry, black plum); vegetables (e.g. cleome, egusi gourd, bitter gourd, amaranth); cereals (e.g. fonio, maize), legumes (e.g. doyi or kersting’s groundnut, soybean, cowpea). We pay special attention to the conservation of orphan crops and crop wild relatives. Despite their vital importance in sustaining livelihoods in Africa, there is a scarcity of data to support policies and development programs. Consequently, our research aims to generate new knowledge that illustrates how adapted plant resources can be instrumental in addressing food security and other basic needs in sub-Saharan Africa. Currently, we maintain a collection of 20,993 accessions of fruits, vegetables, cereals, and legumes from more than ten countries. Our goal is to evolve into the Genetic Resources Conservation Centre of Benin and West Africa, with a target of reaching 30,000 vegetable accessions by 2030. Additionally, our investigations recognize the role of traditional knowledge systems in conserving plant genetic resources. Ethnobotanical studies are central to our strategies for the use and conservation of PGR. Through these efforts, we aim to contribute significantly to the sustainable management and utilization of plant genetic resources for the benefit of communities in the region.